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dc.contributor.authorLeal Pinto, Sandra
dc.contributor.authorHerrera Sandoval, Laura
dc.contributor.authorVargas, Leonor
dc.identifier.citationLeal Pinto, S. M., Herrera Sandoval, L. V., & Vargas, L. Y. (2019). In vitro susceptibility of Microsporum spp. and mammalian cells to Eugenia caryophyllus essential oil, eugenol and semisynthetic derivatives. Mycoses, 62(1), 41–50.
dc.description.abstractBackground: Microsporum s p p are keratinophilic dermatophytes that mainly invade the stratum corneum of the skin and hair causing clinical symptoms associated with tinea. Its treatment has several limitations and the search for new active molecules is necessary. Objetive: To evaluate the antifungal and cytotoxic potential of Eugenia caryophyllus essential oil (EO), eugenol, isoeugenol and methylisoeugenol against Microsporum canis, M. gypseum and Vero cells. Methods: The EO was extracted by conventional heating assisted hydrodistillation , the eugenol obtained commercially and the derivatives through Williamson synthesis. Minimal Inhibitory Concentration (MICs), minimum fungicidal concentration, inhibition of radial mycelial growth and germination inhibition were used to evaluate the antifungal activity. In addition, a colorimetric test was conducte d to evaluate cytotoxic activity. Results: MIC and MFC values for all compounds were 62.5 to 500 μg/ml for both of the species of Microsporum evaluated . Also, concentrations of 300 μg/ml of the compounds inhibited 100% of M. canis mycelium. The inhibition of germination was observed after 6 hours of treatment (11.86 ±3.46 -85.31 ±0 %). No cytotoxicity was observed in Vero cells (CC50 > 105 μg/ml), whereas terbinafin e showed CC50 31.00±0.61 μg/ml. Conclusions: Our study indicates an interesting bioactivity of isoeugenol and methylisoeugenol against M. canis, M. gypseum and mammalian
dc.rightsAtribución-NoComercial-SinDerivadas 2.5 Colombia*
dc.rightsAtribución-NoComercial-SinDerivadas 2.5 Colombia*
dc.titleIn vitro susceptibility of Microsporum spp and mammalian cells to Eugenia caryophyllus essential oil, eugenol and semisynthetic derivativesspa
dc.subject.keywordMicrosporum canisspa
dc.subject.keywordMicrosporum gypseumspa
dc.coverage.campusCRAI-USTA Bogotáspa
dc.relation.referencesGómez E, Crespo V, Martínez L. Dermatofitosis. Piel. 2016;31:546
dc.relation.referencesZhan P, Liu W. The Changing Face of Dermatophytic Infections Worldwide. Mycopathologia . 2017;182(1 -2):77
dc.relation.referencesMejía MA, Santa C, Cadavid M, et al. Estudio etiológico y epidemiológico de las micosis cutáneas en un laboratorio de referencia – Antioquia – Colombia. CES Medicina. 2013; 27(1):7 -19 .spa
dc.relation.referencesBonifaz A. Micología Básica Médica. 5th ed. McGrawHill:108 -158spa
dc.relation.referencesMarchese A, Barbieri R, Coppo E, et al. Antimicrobial activity of eugenol and essential oils containing eugenol: A mechanistic viewpoint. Crit Rev Microbiol. 2017;43(6):668
dc.relation.referencesLeem HH, Kim EO, Seo MJ, Choi SW. Anti -oxidant and anti -inflamatory activities of eugenol and its derivatives from clove (Eugenia caryophyllata Thunb.). J Korean Soc Food Sci Nutr. 2011 ; 40:1361 –
dc.relation.referencesMedeiros A, Lima EO, Leite de Souza E, Formiga MF, Nogueira V, Medeiros IA . Inhibitory effect of b -pinene, a - pinene and eugenol on the growth of potential infectious endocarditis causing Gram -positive bacteria. Rev Bras Cienc Farm . 2007;43(1):121 –127spa
dc.relation.referencesUpadhyay A, Arsi K, Wagle BR, et al. Trans -Cinnamaldehyde, Carvacrol, and Eugenol Reduce Campylobacter jejuni Colonization Factors and Expression of Virulence Genes in Vitro. Front Microbiol. 2017;25:713 doi: 10.3389/
dc.relation.referencesYogalakshmi B, Viswanathan P, Anuradha CV. Investigation of anti - oxidant, anti -in ammatory and DNA -protective properties of eugenol in thioacetamide -induced liver injury in rats. Toxicology . 2010;268(3):204 –
dc.relation.referencesDe Oliveira F, Moura J, de Oliveira E. Investigation on mechanism of antifungal activity of eugenol against Trichophyton rubrum. Med Mycol. 2013;51(5):507 –513. 14. Gupta A, Tu L. Dermatophytes: Diagnosis and treatment. J Am Acad Dermatol. 2006;54(6):1050 -
dc.relation.referencesPinto E, Vale -Silva L, Cavaleiro C, Salgueiro L. Antifungal activity of the clove essential oil from Syzygium aromaticum on Candida, Aspergillus and dermatophyte species. J Med Microbiol. 2009;58(11):1454
dc.relation.referencesEscobar M, Rodríguez K , Ruiz S, Pierdant M, Cerda E, Pozos A. Eugenol Toxicity in Human Dental Pulp Fibroblasts of Primary Teeth. J Clin Pediatr Dent . 2016;40(4):312
dc.relation.referencesNenoff P, Krüger C, Shaller J, Ginter -Hanselmayer G, Shulte - Beerbühl R, Tietz HJ. Mycology – an update Part 2: Dermatomycoses: Clinical picture and diagnostics. J Dtsch Dermatol Ges. 2014;12(9):749
dc.relation.referencesSilva KVS, Lima MIO, Cardoso GN, Santos AS, Silva GS, Pereira FO. Inibitory effects of linalool on fungal pathogenicity of clinical isolates of Microsporum canis and Microsporum gypseum. Mycoses. 2016;60(6): 387
dc.relation.referencesGupta A, Tu L. Dermatophytes: Diagnosis and treatment. J Am Acad Dermatol. 2006;54(6):1050
dc.relation.referencesZuluagaa A, Cáceres D, Arango K, de Bedouta C, Cano L. Epidemiología de la tinea capitis: 19 años de experiencia en un laboratorio clínico especializado en Colombia. Infectio. 2016; 20(4):225 -230spa
dc.relation.referencesKaul S, Yadav S, Dogra S. Treatment of dermatophytosis in elderly, children, and pregnant women. Indian Dermatol Online J. 2017;8(5):310
dc.relation.referencesGinter -Hanselmayer G, Gupta A. Itraconazole in the Treatment of Tinea Capitis Caused by Microsporum canis: Experience in a Large Cohort. Pediatr Dermato. 2004;21(4): 499 –
dc.relation.referencesMoriello K, Coyner K, Susan Paterson S, Mignon B. Diagnosis and treatment of dermatophytosis in dogs and cats. Vet Dermatol. 2017;28(3):266 –
dc.relation.referencesChaieb K, Hajlaoui H, Zmantar T, et al. The Chemical Composition and Biological Activity of Clove Essential Oil, Eugenia caryophyllata (Syzigium aromaticum L. Myrtaceae): A Short Review. Phytother Res. 2007; 21(6):501 –
dc.relation.referencesGülçin I. Antioxidant activity of eugenol: a structure –activity relation - ship study. J Med Food. 2011;14(9):975 –
dc.relation.referencesHan X, Parker LT. Anti -inflammatory activity of clove (Eugenia caryophyllata) essential oil in human dermal fibroblasts. Pharm Biol. 2017;55(1):1619
dc.relation.referencesMahakittikun V, Soonthornchareonnon N, Foongladda S, Boitano JJ, Wangapai T, Ninsanit P. A preliminary study of the acaricidal activity of clove oil, Eugenia caryophyllus. Asian Pac J Allergy Immunol. 2014;32(1):46
dc.relation.referencesSeixas FJ, da Franca Rodrigues KA, Guerra de Oliveira R, et al. Synthesis and In Vitro Anti Leishmania amazonensis Biological Screening of Morita -Baylis -Hillman Adducts Prepared from Eugenol, Thymol and Carvacrol. Molecules 2016;21(11):1483 doi:10.3390/
dc.relation.referencesCooping LG, Duke SO. Natural product that have been used commercially as crop protection agents. Pest Manag Sci . 2006;63(6):6303
dc.relation.referencesLee SJ, Han JI, Lee GS, et al. Antifungal effect of eugenol and nerolidol against Microsporum gypseum in a guinea pig model. Biol Pharm Bull. 2007;30(1):184
dc.relation.referencesRana IS, Rana AS, Rajak RC. Evaluation of antifungal activity in essential oil of the Syzygium aromaticum (L.) by extraction, purification and analysis of its main component eugenol. Braz J Microbiol. 2011; 42(4):269
dc.relation.referencesGayoso CW, Lima EO, Oliveira VT, et al. Sensitivity of fungi isolated from onichomicosis to Eugenia caryophyllata essential oil and eugenol. Fitoterapia. 2005;76(2):247 –
dc.relation.referencesDe Paula SB, Bartelli TF, Di Raimo V, et al. Effect of Eugenol on Cell Surface Hydrophobicity, Adhesion, and Biofilm of Candida tropicalis and Candida dubliniensis Isolated from Oral Cavity of HIV -Infected Patients. Evidence -Based Complementary and Alternative Medicine. 2014; doi: 10.1155/2014/
dc.relation.referencesPavela R. Acude, synergistic and antagonistic effect of some aromatic compound on the Spodoptera littoralis Boisd. (Lep. Noctuidae) larvae. Ind Crops Prod. 2014;60: 247
dc.relation.referencesRossi PG, Bao L, Luciani A, et al. (E) -Methylisoeugenol and Elemicin: Antibacterial Components of Daucus carota L. Essential Oil against Campylobacter jejuni. J Agric Food Chem. 2007;55(18): 7332−7336spa
dc.relation.referencesBaldo A, Monod M, Mathy A, et al. Mechanisms of skin adherence and invasion by dermatophytes. Mycoses. 2011; 55(3):218
dc.relation.referencesBaldo A, Tabart J, Vermout S, et al. Secreted subtilisins of Microsporum canis are involved in adherence of arthroconidia to feline corneocytes. J Med Microbiol. 2008; 57(9):1152 -1156spa
dc.relation.referencesBinh LT, Beaufay C, Trong ND, et al. In Vitro Anti -Leishmanial Activity of Essential Oils Extracted from Vietnamese Plants. Molecules. 2017; 22(7):1071. doi: 10.3390/
dc.relation.referencesPrashar A, Locke IC, Evans CS. Cytotoxicity of clove (Syzygium aromaticum) oil and its major components to human skin cells. Cell Prolif. 2006;39(4):241
dc.relation.referencesFDA. US Food Drug Administration. Guidance for Industry Concerns Related to the use of Clove Oil as an Anesthetic for Fish, Guideline No 150. S. Department of Health and Human Services Food and Drug Administration Center for Veterinary Medicine. cement/guidanceforindustry/ucm052520.pdf accessed october
dc.type.categoryGeneración de Nuevo Conocimiento: Artículos publicados en revistas especializadas - Electrónicosspa

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