Determinación de la mutagenicidad de sustancias migradas desde recipientes plásticos de bajo costo comercializados en Villavicencio (Meta) mediante test Ames

Alvarez Ruiz, Alejandro; Novoa Bermudez, José Mauricio

dc.contributor.advisor	Méndez Leal, María Alexandra	spa
dc.contributor.advisor	Rojas Reina, Christian José	spa
dc.contributor.author	Alvarez Ruiz, Alejandro	spa
dc.contributor.author	Novoa Bermudez, José Mauricio	spa
dc.date.accessioned	2020-07-10T15:12:15Z	spa
dc.date.available	2020-07-10T15:12:15Z	spa
dc.date.issued	2020-06-18	spa
dc.identifier.citation	Alvarez, A., & Novoa, M. (2020). Determinación de la mutagenicidad de sustancias migradas desde recipientes plásticos de bajo costo comercializados en Villavicencio (Meta) mediante test Ames. Tesis de pregrado, Universidad Santo Tomás, Villavicencio.	spa
dc.identifier.uri	http://hdl.handle.net/11634/27981
dc.description	La presencia de sustancias mutagénicas en los materiales plásticos usados en la vida cotidiana, particularmente en aquellos destinados al contacto con alimentos y bebidas, puede llegar a representar una problemática para salud pública y el medio ambiente, debido a los efectos adversos en la salud humana relacionados principalmente con daños al ADN, problemas cardiovasculares, afectaciones en la fertilidad, alteraciones neurológicas, entre otras enfermedades, así como la degradación del suelo, fuentes hídricas subterráneas y la alteración de receptores ambientales como microorganismos, plantas y animales. Desde esta problemática, la presente investigación evaluó el índice de mutagenicidad de agua en contacto con recipientes plásticos de bajo costo, empleados para el transporte, almacenamiento y calentamiento comercializados en la ciudad de Villavicencio Meta, en función del uso y tipo de recipiente. Se utilizó el test Ames como método de identificación del índice de mutagenicidad de las muestras obtenidas. Para el tratamiento estadístico de los datos se utilizó la prueba t de Student y el análisis de varianza ANOVA con prueba HSD de Tukey. El test Ames se efectuó con Salmonella typhimurium, siguiendo el protocolo descrito por Ames et al. (1975). El test incluyó controles positivos y negativos y fue llevado a cabo sin activación metabólica. Todas las pruebas fueron aplicadas en triplicado. Las tasas de reversión espontánea obtenidas se mantuvieron dentro de los valores normales. Se obtuvieron índices de mutagenicidad que oscilaron entre 0,74 y 1,52, valores considerados como "No mutagénicos" o "Ligeramente mutagénicos" según la escala de análisis. Se pudo inferir que el índice de mutagenicidad puede llegar estar relacionado con el desgaste del material al que se exponen estos recipientes, debido a la relación entre el número de calentamientos aplicado y el índice de mutagenicidad obtenido. De igual forma, se comprobó estadísticamente la influencia de las características físicas del recipiente en el aumento en el índice de mutagenicidad, considerando como más adecuados los recipientes fabricados con polipropileno, transparentes y que en su etiqueta se enuncien como libres de BPA.	spa
dc.description.abstract	The presence of mutagenic substances in the plastic materials used in everyday life, specifically in those intended for contact with food and beverages, may become a problem for public health and the environment, due to adverse effects on the human health mainly related to DNA damage, cardiovascular problems, fertility disorders, neurological disorders, among other diseases, as well as soil degradation, underground water sources and the alteration of environmental receptors such as microorganisms, plants and animals. Due to this problem, the present investigation evaluated the mutagenicity index of water in contact with low-cost plastic containers, used for transport, storage and heating, commercialized in the city of Villavicencio (Meta), in contrast with use and type of container. The Ames test was used as a method of identifying the mutagenicity index of the obtained samples. For the statistical treatment of the data, the Student's t-test and the analysis of variance ANOVA with the Tukey's HSD test were used. The Ames test was carried out with Salmonella typhimurium, following the protocol described by Ames et al. (1975) The test included positive and negative controls and was carried out without metabolic activation. All tests were applied in triplicate. The spontaneous reversion rates remained within normal values. Mutagenicity index ranged between 0.74 and 1.52, obtaining values labeled as "Non-mutagenic" or "Slightly mutagenic" according to the analysis scale. it can be inferred that mutagenicity index can be related to the normal wear to which these recipients are exposed, based on the relationship between the number of heating applied and the mutagenicity index obtained. Likewise, the influence of the physical characteristics of the container on the increase in the mutagenicity index was statistically verified, considering that containers made with polypropylene, transparent and labeled as BPA-free, are the most appropriate.	spa
dc.format.mimetype	application/pdf	spa
dc.language.iso	spa	spa
dc.publisher	Universidad Santo Tomás	spa
dc.rights	Atribución-NoComercial 2.5 Colombia	*
dc.rights	Atribución-NoComercial 2.5 Colombia	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc/2.5/co/	*
dc.title	Determinación de la mutagenicidad de sustancias migradas desde recipientes plásticos de bajo costo comercializados en Villavicencio (Meta) mediante test Ames	spa
dc.description.degreename	Ingeniero Ambiental	spa
dc.publisher.program	Pregrado de Ingeniería Ambiental	spa
dc.publisher.faculty	Facultad de Ingeniería Ambiental	spa
dc.subject.keyword	Ames Test	spa
dc.subject.keyword	Mutagenicity	spa
dc.subject.keyword	Plastic Containers	spa
dc.subject.keyword	Migration	spa
dc.subject.lemb	Mutagenicidad	spa
dc.subject.lemb	Propiedades biológicas	spa
dc.subject.lemb	Materiales plásticos	spa
dc.subject.lemb	Salud pública	spa
dc.subject.lemb	Alimentos - Empaques	spa
dc.subject.lemb	Ingeniería ambiental	spa
dc.subject.lemb	Tesis y disertaciones académicas	spa
dc.type.local	Trabajo de Grado	spa
dc.rights.local	Abierto (Texto Completo)	spa
dc.type.version	info:eu-repo/semantics/acceptedVersion
dc.rights.accessrights	info:eu-repo/semantics/openAccess
dc.coverage.campus	CRAI-USTA Villavicencio	spa
dc.contributor.orcid	https://orcid.org/0000-0003-4960-4211	spa
dc.contributor.orcid	https://orcid.org/0000-0002-1044-3329	spa
dc.contributor.googlescholar	http://scholar.google.com/citations?user=GiWWc18AAAAJ&hl=en	spa
dc.contributor.cvlac	https://scienti.minciencias.gov.co/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0001528488	spa
dc.contributor.cvlac	http://scienti.colciencias.gov.co:8081/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0000089317	spa
dc.description.domain	http://www.ustavillavicencio.edu.co/home/index.php/unidades/extension-y-proyeccion/investigacion	spa
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dc.relation.references	Xue, C., & Shu, J. (2013). Migration study of bisphenol A from tea bottle of polycarbonate plastic to tea water. Advanced Materials Research, 821–822, 929–932. https://doi.org/10.4028/www.scientific.net/AMR.821-822.929	spa
dc.relation.references	Zuluaga, M., Valencia, A., & Ortíz, I. (2009). Efecto genotóxico y mutagénico de contaminantes atmosféricos. Medicina UPB, 28(1), 33–41.	spa
dc.rights.coar	http://purl.org/coar/access_right/c_abf2
dc.subject.proposal	Test Ames	spa
dc.subject.proposal	Mutagenicidad	spa
dc.subject.proposal	Recipientes plásticos	spa
dc.subject.proposal	Migración	spa
dc.identifier.reponame	reponame:Repositorio Institucional Universidad Santo Tomás	spa
dc.identifier.instname	instname:Universidad Santo Tomás	spa
dc.type.category	Formación de Recurso Humano para la Ctel: Trabajo de grado de Pregrado	spa
dc.type.coar	http://purl.org/coar/resource_type/c_7a1f
dc.description.degreelevel	Pregrado	spa
dc.identifier.repourl	repourl:https://repository.usta.edu.co	spa
dc.type.coarversion	http://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.drive	info:eu-repo/semantics/bachelorThesis