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IN VITRO ANTIMICROBIAL ACTIVITY OF ETANOLIC PROPOLIS EXTRACTS FROM SANTANDER AGAINST ENTEROCOCCUS FAECALIS

dc.contributoren-US
dc.contributores-ES
dc.creatorHerrera Sandoval, Laura Viviana
dc.creatorPisciotti Ortega, Marcos Humberto
dc.creatorRamos Monsalve, Otoniel
dc.creatorNeira Fuentes, Laura Fernanda
dc.creatorPinzón Joya, Julio Roberto
dc.creatorHerrera Becerra, Adriana Victoria
dc.creatorSoto Salcedo, José Antonio
dc.date2012-07-01
dc.identifierhttp://revistas.ustabuca.edu.co/index.php/USTASALUD_ODONTOLOGIA/article/view/1119
dc.identifier10.15332/us.v11i2.1119
dc.descriptionObjective: to evaluate the antimicrobial activity of an ethanolic propolis extract from Santander province against Enterococcus faecalis.Methods: an experimental In vitro study was made. Starting from pristine propolis collected in the city of Lebrija (Santander), an ethanolic extract was obtained using a Soxhlet apparatus. The antimicrobial activity was measured using the macro dilution tube technique by counting the Colony Forming Units (CFU) per mL after 48 hours. Propolis solutions with concentrations in the range from 100 to 0.19 mg/mL were evaluated. Tubes containing dilutions of the antibiotic norfloxacin and solvent (96% ethanol) were used as reference and control.Results: the propolis solutions within the range from 100 mg/mL to 50 mg/mL inhibited 100% growing of E. faecalis. Propolis extract showed an inhibitory concentration 50 (IC50) of 1.19 mg/mL and an inhibitory concentration 90 (IC90) of 7.92 mg/mL respectively. Norfloxacin on the other hand was more effective with IC50 and IC90 of 0.06 and 2.39 µg/mL respectively. Ethanol showed non-antimicrobial activity at the concentration present within the extracts.Conclusion: with further studies support it would be possible to suggest the use of propolis from Santander as an alternative of irrigation and medication in endodontics because of its powerful effect against E. faecalis considering it is the most frequent pathogen found in persistent infections.en-US
dc.descriptionObjetivo: evaluar la actividad antimicrobiana de un extracto etanólico de propóleo de Santander sobre Enterococcus faecalis.Materiales y métodos: se realizó un estudio experimental In vitro. A partir de un propóleo crudo del municipio de Lebrija (Santander), se obtuvo un extracto etanólico a través del método Soxhlet. La actividad antimicrobiana se determinó con una prueba de macrodilución en tubo y recuento de Unidades Formadoras de Colonias (UFC) por mL luego de 48 horas. Se evaluaron concentraciones de propóleo desde 100 hasta 0,19 mg/mL, como control se incluyeron diluciones del disolvente (etanol 96%) y se usó como medicamento de referencia la norfloxacina.Resultados: se determinó que concentraciones de propóleo de 100 mg/mL y 50 mg/mL inhibieron 100% de crecimiento de E. faecalis. El extracto de propóleo mostró Concentración Inhibitoria 50 (CI50) de 1,19 mg/mL y Concentración Inhibitoria 90 (CI90) de 7,92 mg/mL. Mientras que la norfloxacina fue más efectiva con CI50 y CI90 de 0,06 y 2,39 µg/mL. El etanol no mostró efecto antimicrobiano a las concentraciones presentes en el extracto.Conclusión: con el apoyo de estudios complementarios, es posible proponer al propóleo santandereano como una alternativa de irrigación y medicación en endodoncia, dado el potente efecto frente a E. faecalis siendo este el patógeno endodóntico más frecuente en infecciones persistentes.[Herrera LV, Piscioti MH, Ramos O, Neira LF, Pinzón JR, Herrera AV, Soto JA. Actividad antimicrobiana In Vitro de un propóleo de Santander sobre Enterococcus faecalis. Ustasalud 2012; 11: 73 - 78]es-ES
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dc.languagespa
dc.publisherUniversidad Santo Tomás Seccional Bucaramangaes-ES
dc.relationhttp://revistas.ustabuca.edu.co/index.php/USTASALUD_ODONTOLOGIA/article/view/1119/918
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dc.rightsCopyright (c) 2018 UstaSaludes-ES
dc.sourceUstaSalud; Vol. 11, Núm. 2 (2012); 73-78es-ES
dc.source1692-5106
dc.source10.15332/us.v11i2
dc.subjectPropolis; Antibacterial agents; Enterococcus faecalisen-US
dc.subjectActividad bactericida; Endodoncia; Enterococcus faecalises-ES
dc.titleACTIVIDAD ANTIMICROBIANA IN VITRO DE UN PROPÓLEO DE SANTANDER SOBRE ENTEROCOCCUS FAECALISes-ES
dc.titleIN VITRO ANTIMICROBIAL ACTIVITY OF ETANOLIC PROPOLIS EXTRACTS FROM SANTANDER AGAINST ENTEROCOCCUS FAECALISen-US
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typeArtículo revisado por pareses-ES


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