Actividad larvicida de aceites esenciales y extractos de plantas colombianas frente a Culex quinquefasciatus (Díptera: Culicidae)
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Universidad Santo Tomás. Seccional Bucaramanga
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Los aceites esenciales y extractos de plantas,cultivadas de forma orgánica en la finca El Limonal Piedecuesta-Santander, Colombia, obtenidos por hidrodestilación asistida por microondas y percolación, se caracterizaron por cromatografía de gases acoplada a espectrometría de masas. La actividad insecticida se evaluó frente a larvas en tercer instar de Culex quinquefasciatus y en la enzima acetilcolinesterasa. La toxicidad se determinó en el modelo del pez cebra (Danio rerio). De ellos, el aceite esencial de Ruta graveolens fue el que presentó la mejor actividad larvicida, con CL50 7.20±0.19 μg/mL, una moderada inhibición de la enzima con IC50 44.98±1.1 μg/mL y moderada toxicidad con CL50 13.33±0.59 mg/L.
Essential oils and extracts obtained by microwave hydrodistillation and percolation from plants organically cultivated in the farm El Limonal Piedecuesta-Santander, Colombia, were characterized by Gas Chromatography coupled with Mass Spectrometry. The insecticidal activity against third instar larva of Culex quinquefasciatus and the inhibition activity of acetylcholinesterase of those compounds were studied. Also, the toxicity was evaluated against the zebrafish (Danio rerio). Our results show that the Ruta graveolens essential oil had the highest larvicidal activity with a LC50 7.20±0.19 μg/mL, a moderate inhibition over the enzyme with an IC50 44.98±1.1 μg/mL and a moderate toxicity with a LC50 13.33±0.59 mg/L.
Essential oils and extracts obtained by microwave hydrodistillation and percolation from plants organically cultivated in the farm El Limonal Piedecuesta-Santander, Colombia, were characterized by Gas Chromatography coupled with Mass Spectrometry. The insecticidal activity against third instar larva of Culex quinquefasciatus and the inhibition activity of acetylcholinesterase of those compounds were studied. Also, the toxicity was evaluated against the zebrafish (Danio rerio). Our results show that the Ruta graveolens essential oil had the highest larvicidal activity with a LC50 7.20±0.19 μg/mL, a moderate inhibition over the enzyme with an IC50 44.98±1.1 μg/mL and a moderate toxicity with a LC50 13.33±0.59 mg/L.
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Aceites esenciales, actividad larvicida, bioensayos en embriones cebra, Culex quinquefasciatus, extractos, inhibidores de acetilcolinesterasa, Acetylcholinesterase inhibitors, Culex, quinquefasciatus, essential oils, extracts, larvicidal activity, zebrafish embryos bioassays
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