Acción anitmicrobiana de allium sativum sobre enterococcus faecalis sembrado dientes humanos in vitro

Abstract

One of the objectives in endodontic therapy is the reduction of pathogen agents as minimal as possible, without harm or secondary effects on periradicular tissues. Some authors confirm that altering the environment of microorganisms, they can be reduced. To that end, natural substances have properties that can help to conventional therapy, such as antimicrobial, antiinflamatory, besides they are easy to obtain and manipulate; that can reduce the presence of Enterococcus faecalis, one of the most resistant and persistent specie in root canals. Objective: to evaluate the antimicrobial effect of Allium sativum extract in the growth of Enterococcus faecalis inoculated in human teeth in vitro. Method: obtaining aqueous pure extract of A. sativum. 54 teeth prepared and sterilized, they were inoculated with Enterococcus faecalis, divided in 3 groups of 18 teeth each one, Group 1 treated with extract of A. sativum, Group 2 treated with saline solution, group 3 treated with sodium hypochlorite. Times evaluated 1, 5, 10, 15, 30, 60 minutes. Results: The extract of A. sativum showed bacterial reduction in all the times evaluated in a range of 42,9 – 60,6%: sodium hypochlorite had 100% of bacterial reduction and saline solution had no antimicrobial activity. Conclusions: the extract of A. sativum had antimicrobial activity against E. faecalis in all the times evaluated; there was more bacterial reduction in higher time of exposition, being 60,6% in 60 minutes.