Efecto antibacteriano de la biocerámica masilla y mineral trióxido agregado blanco en un modelo de biopelícula bacteriana in vitro. Estudio piloto

Abstract

Introduction: The purpose of the study is to determine the antibacterial effect of the ERRM® root repair material compared to ProRoot White® (MTA) in an in vitro bacterial biofilm model using Confocal Microscopy (CLSM). Method: Direct contact method was performed with a dilution of the materials at a concentration of 125 mg / 1mL, an in vitro biofilm model was made in synthetic hydroxyapatite discs incubated under anaerobic conditions and evaluated at 24, 48 and 72 hours It was analyzed by CLSM evaluating cell viability, the Kolmogorov-Smirnov test was performed to evaluate the normality of the data distribution, the statistical significance was established when the p-value was less than 0.05. Results: The percentage of dead cells as follows: For the ERRM® at 24 hours it was 5%, at 48 hours at 11% and at 72 hours at 10%, which indicates that over time the antibacterial effect was better. In turn, the MTA showed percentages of 12%, 9% and 6% at 24, 48 and 72 hours respectively, which indicates that over time the antibacterial effect decreased, but there are no differences. Conclusions: 1. An in vitro biofilm model was developed. 2. A modified method was developed to evaluate the behavior of the materials in the biofilm. 3. Neither of the two materials had antibacterial efficacy against the biofilm.