Análisis del riesgo microbiológico del aire en dos laboratorios de la Universidad Santo Tomás Sede Villavicencio Campus Aguas Claras.

Abstract

The determination of microorganisms in indoor air becomes important when analyzing the health impact of people who frequent internal environments, since air is a means of dispersion of many pathogenic microorganisms such as bacteria, fungi and yeasts. In the present paper the possible exposure to pathogenic microorganisms present in the air was identified and the level of health risk that can be generated to users who make use or carry out academic activities in the Biology and Microbiology and Toxicology and Biotechnology laboratories was determined at Santo Tomás University in Villavicencio, Aguas Claras campus. The methodology used to achieve the objective of the research was divided into 3 phases: 1 quantify and identify the genus of bacteria and fungi and yeasts present in the air of the laboratories under study, 2 classify the microorganisms present in the laboratories subject to study according to their level of risk in human health and 3 establish biosafety protocol and recommendations for mitigation of risk in infrastructure for the protection of users in these two enclosures. In the samples, a greater quantity of Gram positive bacteria (61%) than Gram negative bacteris (39%) were obtained, identifying the genera Staphylococcus sp, Bacillus sp, Bacillus cereus and Bacillus subtilis as Gram positives and Enterobacter aerogenes, Escherichia coli, Klebsiella pneumoniae, Klebsiella oxytoca, Salmonella sp, Shigella sonnei and Yersinia pseudotuberculosis as Gram negative. In fungi it was determined that the isolated genera with the highest frequency of recognition in the two laboratories under study were Rhizopus sp and Aspergillus sp, unlike Cladosporium sp, Mucor sp and Penicillium sp, which presented less frequency.