Efecto de croton lechleri sobre la viabilidad, proliferación y citotoxicidad en osteoblastos humanos estudio in vitro

Abstract

The Croton Lechleri, is a tree which is extracted from a red sap (known as Sangre de Drago), to which are attributed many therapeutic properties highlighting its healing action, which is why it is important to know their effect on osteoblasts humans. Objective: To determine the effect of Croton Lechleri, "Sangre de Drago®" on the viability proliferation and cytotoxicity in human osteoblasts "in vitro". Method: quasiexperimental study "In Vitro". Tests were conducted to determine viability, proliferation and cytotoxicity of osteoblasts in the presence Sangre de Drago® in different concentrations, using MTT and flow cytometry FACS techniques. Results: viability of the osteoblasts was observed in the presence of different concentrations Sangre de Drago® and Annexin V showed no cytotoxic effect on osteoblasts. Conclusions: Croton lechleri "Sangre de Drago®" was not cytotoxic to the cell line of osteoblasts allowing cell viability.