Citotoxicidad del mta y biocerámico sobre células madre de la pulpa dental

Abstract

Objective: to compare the cytotoxicity of MTA Proroot® and EndoSequence® putty and paste, in human dental pulp stem cells. Methodology: In vitro experimental study. The cells were transported in a T75 bottle, from the Universidad del Bosque to the National University of Colombia. The cells were seeded in 6-well plates, which previously had 3mg of each of the materials. The cell count was performed with a hemocytometer and triphan blue under the inverted microscope. The counts were made at 24, 48 and 72 hours in duplicate. Results: The ANOVA statistical test was applied and it was found that the number of living cells was greater for the putty at 24 and 72 hours. In the case of the MTA presented the lowest number of living cells during the exposure times evaluated (24, 48, 72 hours). Conclusion: The bioceramic material is less cytotoxic than MTA, this is a safe material for applications that involve contact with adjacent tissue.