Evaluating Colombian Clostridium spp. strains’ hydrogen production using glycerol as substrate
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Abstract
Background: The ability for hydrogen production of 13 native strains of Clostridium spp. strains
isolated from Colombian soil was evaluated using glycerol substrate. Glycerol to hydrogen conversion
was investigated using a batch fermentation reactor and industrial glycerol source (50 g.l-1 , pH 7.00).
Results: The results were quantified regarding acids, hydrogen, biomass and solvent production. The
selected strain gave good hydrogen over production output at 14.4 mmol H2.l-1
, productivity 0.3167 mg
H2.h-1 l - 1 culture mediumand yield 0.1962 mol H2.mol-1
glycerol. A further fermentation assay a 4.0 liter
batch reactor let to being 0.26 mg.l-1
.h-1 after 18 hrs of fermentation. Logistic model, Luedeking-Piret
model and Luedeking-Piret modified models were used for modeling changes in cell growth, hydrogen
production and substrate consumption (Correlation coefficients R2
= 0.95 for biomass substrate, R2 = 0.77 hydrogen production).
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Atribución-NoComercial-CompartirIgual 2.5 Colombia