The effect of single-file reciprocating systems on Substance P and Calcitonin gene-related peptide expression in human periodontal ligament
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2012-09-11
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Abstract
Aim To quantify the effect of two single-file reciprocating root canal preparation systems on Substance P
(SP) and Calcitonin gene-related peptide (CGRP)
expression in healthy human periodontal ligament
(PDL).
Methodology Forty PDL samples were obtained
from healthy premolars where extraction was indicated for orthodontic reasons. Prior to extraction, 20
of these premolars were divided equally in two
groups, and then, root canals were prepared using
one of two different single-file systems: WaveOne and
Reciproc. Ten premolars were prepared with hand
files and served as a positive control group. The
remaining 10 premolars where extracted without
treatment and served as a negative control group. All
PDL samples were processed, and SP and CGRP were
measured by radioimmunoassay.
Results Greater SP and CGRP expression were found
in the hand instrumentation group (1.220 pmol SP and 0.084 pmol CGRP per mg of PDL), followed by
the WaveOne group (0.908 pmol SP and 0.046 pmol
CGRP per mg of PDL) and the Reciproc group
(0.511 pmol SP and 0.022 pmol CGRP per mg of
PDL). The lower SP and CGRP values were associated
with the intact control group (0.453 pmol SP and
0.018 pmol CGRP per mg of PDL). The Kruskal–Wallis
test revealed significant differences between groups
(P < 0.001). Post hoc Tukey HSD tests revealed significant differences in SP and CGRP expression between
intact teeth in the control group and all the other
groups (P < 0.001) except with the Reciproc group
(P = 0.165 and P = 0.42 for SP and CGRP, respectively). Hand instrumentation was associated with
significant differences with all the other groups
(P < 0.001). Differences between the WaveOne and
Reciproc groups were also significant (P < 0.001).
Conclusion Substance P and CGRP expression in
PDL cells increased when teeth were prepared with
WaveOne as well as with hand instrumentation. Reciproc maintained SP and CGRP levels in line with the
negative control group.
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