Proliferation of 3T3 fibroblasts in a spinner type Reactor on microcarriers of fibrin-alginate
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2015-01
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Abstract
- The microcarriers have become an alternative of
cell culture in order to increase the contact area per unit volume
of culture medium. The aim of this work is the fabrication of
microcarriers from a mixture of sodium alginate and human
blood plasma, to be used as a fibroblast support during
proliferation in bioreactors. Microcarriers were obtained by
spraying through a coaxial nozzle over calcium chloride. PolyL-Lisine (PLL) and fibrinogen were adsorbed on the surface of
microcarriers. The microcarriers were observed by atomic
force microscopy (ATM) and scanning electronic microscopy
(SEM). Adhesion of 3T3 fibroblasts was evaluated over the
microcarriers surface. Microspheres between 150 μm and
500μm of diameter were obtained, it was evaluated the cellular
adhesion and a first-order kinetics was adjusted with a constant
k = 0.007 min-1
. Celullar adhesion was observed on the
microcarriers using confocal microscopy and fluorescence
microscopy. Fibroblasts grown in the reactor showed that it is
possible to reduce the doubling time from 23.4 until 16.3 h.
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