Study on the inhibitory effect of furafylline and troleandomycin in the7-methoxyresorufin-O-demethylase and nifedipine oxidase activitiesin hepatic microsomes from four poultry species usinghigh-performance liquid chromatography coupled with fluorescenceand ultraviolet detection
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2018-10-22
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Abstract
tThe present study reports the in vitro studies with furafylline and troleandomycin (TAO) as specificinhibitors of activities 7-methoxyresorufin-O-demethylase (MROD) and nifedipine oxidase, catalyzed bycytochrome P450 1 A2 (CYP1 A2) and 3A4 human enzymes, respectively, in hepatic microsomes of quail,duck, turkey and chicken. The results suggest that in chicken and quail the MROD activity is carried out byorthologs CYP1 A4 and 1 A5, meanwhile in duck and turkey by a CYP1 A5 ortholog. The nifedipine oxidaseactivity is carried out by orthologs of the CYP3A family in the four bird species. The use of furafyllineand TAO significantly decreased these activities (P < 0.05) and suggested that the biotransformation ofresorufin methyl ether (RME) may be related to more than one avian ortholog.
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Murcia, H., Cruz, A., León, J., & González-Curbelo, M. Á. (2018). Study on the inhibitory effect of furafylline and troleandomycin in the7-methoxyresorufin-O-demethylase and nifedipine oxidase activitiesin hepatic microsomes from four poultry species usinghigh-performance liquid chromatography coupled with fluorescenceand ultraviolet detection. Bogotá: doi:/10.1016/j.jpba.2018.10.031
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