Chemical salivary composition and its relationship with periodontal disease and dental calculus
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2015-06
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Abstract
Aim: To determine the relationship between the chemical composition of saliva, periodontal
disease and dental calculus. Methods: An observational analytical cross-sectional study was
conducted with patients over 55 years of age. Ethical principles of autonomy and risk protection
were applied according to the international standards. Sociodemographic and diagnosis variables
(presence of dental calculus and periodontal status) were considered to measure salivary
concentrations of glucose (by the glucose oxidase/peroxidase method, amylase (by the colorimetric
test), urea (by the amount of indophenol), total protein (by the Bradford method) and albumin (by the
nephelometric method). Patients chewed a sterile rubber band and 3 mL of stimulated saliva were
collected. The samples were stored at -5 °C, centrifuged at 2,800 rpm for 10 min, and the supernatant
was removed and stored at -20 °C. Data were presented as frequencies and proportions for
qualitative variables and measures of central tendency and dispersion for quantitative variables.
Data were analyzed by either analysis of variance or Kruskal Wallis test . A p value <0.05 was
considered statistically significant. Results: Significant relationships were observed between the
concentration of salivary urea and periodontal status (p = 0.03) and the presence of dental calculus
and urea (p = 0.04) was demonstrated. Conclusions: A relationship between the salivary urea
concentration and the presence of periodontal disease and dental calculus is suggested.
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Atribución-NoComercial-CompartirIgual 2.5 Colombia