Degradación de polietileno tereftalato (PET) generado en el corredor vial comercial de la Cuncia por medio de Pseudomonas aeruginosa.

Abstract

Currently, plastic and PET bottles are being manufactured in an uncontrolled manner in order to provide a necessity to people, but this is generating a consequence since there is no controlled management of the final disposal for its use or degradation (Gómez Serrato, 2016). Pseudomonas aeruginosa is a gram-negative, aerobic, oxidase-positive bacterium that has the characteristic of adapting quickly to the habitat where it is found and supplying itself with carbon and nitrogen sources for its nutrition. This degree work evaluates the degradation efficiency of polyethylene terephthalate (PET) by means of a laboratory count of Pseudomonas aeruginosa seeded in cetrimide agar added with PET at different concentrations, which was carried out with an experimental and descriptive methodology in 3 phases, where the characterization of the solid waste generated in the commercial businesses of the road corridor of La Cuncia located at km 14 via Villavicencio - Acacias begins, After this, phase two (2) begins, where the study is carried out under laboratory conditions, where the processes of determining the growth curve of Pseudomonas aeruginosa by spectrophotometry at different wavelengths, determination of the bacterial concentration by counting the CFU plate and evaluation of the bacteria degrading PET at different concentrations by means of descriptive statistical analysis are carried out. For the third phase, the results were socialized to the commercial establishments of the road corridor of La Cuncia, where three (3) bioreactors of P. aeruginosa were implemented, taking into account variables of temperature, pH, initial weight of PET and different bacterial concentrations (106, 107, 108). This experiment was carried out for 10 days and then taken to the laboratory to read the results. As a result of this project, it was found that the highest colony growth occurred at a bacterial concentration of 106 (Hour 34) and 107 (Hour 48) with a microorganism dilution of 106. With respect to the prototype, a PET degradation of 3.5% was achieved in a period of 10 days, which represents a significant result due to the short time in which the prototype was implemented.