Evaluación de la actividad antioxidante e inhibición de la acetilcolinesterasa de extractos etanólicos obtenidos de cáscaras de naranja

Abstract

Colombia is a major producer of citrus worldwide, while fresh trade is less compared to its production, revealing a large domestic consumption. Because of this, large amounts of waste that can be exploited, since they have secondary metabolites such as flavonoids that are compounds having variety of biological properties as, anti-inflammatory, anticancer, antioxidant, antiviral, antibacterial, among other. The main objective of this work was the preparation of compounds of value added flavonoids type, from orange peels. These shells were subjected to ethanolic extraction methods Soxhlet solid-liquid, ultrasound assisted extraction and microwave assisted extraction. Orange peels were worked in a cool dry state. Extracts obtained antioxidant activity through total polyphenol content, measurement of total flavonoids and radical cation ABTS+ was assessed, the In vitro inhibitory activity of the enzyme acetylcholinesterase extracts was analyzed. Soxhlet extraction, had better performance in the samples fresh, the average was 8.9% and the dried shells with an average of 28.4%. The dry samples showed good results in the measurement of total flavonoids and polyphenols with values of 56,9 mg GA / g dry extract and 1,25 mg quercetin / g dry extract respectively. In the antioxidant activity, the extracts obtained by microwaves, for fresh samples were more efficient with an average value of the Total Antioxidant Actvity (TAA) of 150.1 mmol of trolox / kg sample. As inhibition of acetylcholinesterase by microwaves for dry samples, they threw an average inhibitory concentration of 34.1 μg/ mL.