Validación de métodos analíticos para la determinación de nitratos, nitritos, ortofosfatos y nitrógeno amoniacal en aguas, en el laboratorio de SIAMA Ltda.

Abstract

The objective of this job was perform validation of analytic methods to determinate nitrates, nitrites, orthophosphates and ammoniac nitrogen in water. Validation of nitrites, nitrates and orthophosphates was determined by colorimetric methods and ammoniac nitrogen by distillation – titration method. This project is part of fulfillment about one of the requirements established by the norm NTC ISO/IEC: 17025 – 2005 for the laboratory SIAMA LTDA, can be accredited. To develop these validations, was taken as reference the analytic procedures of SIAMA LTDA’s laboratory, the standard method 22 edition for water analysis and the Eurachem guide for validation and determination to the analytics method uncertainty. On the validation process, each one of the methodologies, were established values as: Detection limit, limit of quantification, repeatability, linearity, accuracy, recovery, working range, and uncertainty calculation. It concludes that the valid methods of SIAMA LTDA´s laboratory are adequate to apply in the water’s analysis and make reliable results.