Determinación de la capacidad antioxidante de extractos etanólicos de la Spilanthes americana

Abstract

Natural antioxidants, especially of plant origin, have increased their demand in recent years due to the growing concern of consumers for synthetic antioxidants and their toxicological effects. Due to this, different vegetable products are evaluated as natural antioxidants to preserve and improve the quality of food and life of consumers (Ahmad Shah et al, 20014). These antioxidants are obtained from sources such as fruits, vegetables, herbs and spices and are rich in phenolic compounds (Ghorbani Gorji et al, 2016). The main objective of this work was to determine the antioxidant potential of flavonoid compounds present in ethanol extracts of Spilanthes americana. This herb was subjected to soxhlet solid-liquid extraction methods and ultrasound-assisted extraction, which was worked in the fresh state. Obtained the ethanolic extracts of the Spilanthes americana, the antioxidant activity was evaluated through the content of total flavonoids, total polyphenols and implementation of the radical cation ABTS + method. The extractions employed had a very similar average yield, 1.44% soxhlet extraction and 1.4% ultrasound-assisted extraction. The colorimetric tests performed to determine the presence of total flavonoids and total polyphenols in all ethanolic extracts obtained were positive. The total number of flavonoids and total polyphenols were higher in the extracts obtained by the ultrasound-assisted extraction, showing results of 0.00357 mg quercetin / g dry extract and 0.000687 mg Gallic Acid AG / g dry extract, respectively.