Búsqueda de microorganismos con alto potencial antagonista de Botrytis cinerea, procedentes de manantiales termales y salinos colombianos

Abstract

One of the most limiting problems in the production and postharvest of fruits, vegetables, ornamental, among others, are those produced by the gray mold caused by B. cinerea, which is a phytopathogenic fungus of wide geographic distribution. For the control of this agent, fungicides are used as the first line, some of them causing phenomena of resistance in this phytopathogen and generators of risks to human health and potential risk of environmental contamination. Taking into account this problem, the research and evaluation of halophilic bacteria with B. cinerea antagonistic activity is proposed in this research. The methodology to be developed in this research consists of two stages: (i) selection of a minimum concentration for the manifestation of symptoms and signs of gray mold at 6 ° C and 20 ° C and strawberry fruits; (Ii) isolation and characterization of halophilic microorganisms isolated from saline and thermal springs in Colombia, (iii) evaluation of antagonistic potential of halophilic strains isolated in an in-plant model of tomato fruit, both at 6 ° C In post-harvest conditions) and at temperatures of 20 ° C, (iv) evaluation of the resistance capacity of selected bacteria at different ranges in ultraviolet light, and (v) evaluation in combination with CaCl2 for control of gray mold at temperature Cooling (6 ° C). This study aims to obtain a collection of halophilic microorganisms, characterized phylogenetically and physiologically, that present a high antagonistic activity to be used as biological controllers of B. cinerea.