Evaluación del desempeño del pretratamiento con peróxido de hidrógeno sobre mezclas de biomasas lignocelulosicas (bagazo de caña panelera y hojarasca)

Abstract

In this research, the changes in the mixture of different lignocellulosic biomasses were evaluated after being subjected to a chemical pretreatment, implemented to increase the exposure of the cellulose for its subsequent enzymatic hydrolysis. The pretreatment used is the oxidative pretreatment with hydrogen peroxide (H2O2), on the mixture in proportion (90:10) of bagasse of sugarcane and litter respectively. , Lignocellulosic biomass mixed was carried out in order to evaluate the behavior, with excellent results as a reference, when the sugar cane bagasse is used with this chemical pretreatment. The biomass was characterized initially to determine soluble and insoluble lignin, moisture and ashes and crystallinity index of the lignocellulosic material using x-ray diffraction (XRD). Experimental design 23 was realized using different concentrations of hydrogen peroxide, times and temperature, with triplicate at the center point in the trials (2, 5, and 11) to obtain an increase in the exposure of the Cellulose and greater solubilization of lignin. The results obtained in the pretreatment stage show a good removal of insoluble lignin with a performance of 88.5% with reaction conditions of 80 min, 25 ° C, 7% of hydrogen peroxide and 79.5% % under conditions of 40 min, 25 ° C, 3% of hydrogen peroxide. For soluble lignin with 58.8% removal and conditions of 80 min, 25 ° C, 7% of hydrogen peroxide and 61.67% with conditions of 40 min, 25 ° C, 7% of hydrogen peroxide considered with high solubilization of lignin. The experimental design shows the optimization of the process to reduce costs and time by identifying the significant variables of the model (time, temperature and interaction 1by3) with 95% confidence by means of statistical analysis with the STATISTIC 7.0 software. Analysis of variance and response surfaces, considering the concentration as a non-significant variable.