Preclinical evaluation of collagen type I scaffolds, including gelatin-collagen microparticles and loaded with a hydroglycolic Calendula officinalis extract in a lagomorph model of full-thickness skin wound
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2015-11-23
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Abstract
Previously, we have developed collagen type I scaffolds
including microparticles of gelatin-collagen type I
(SGC) that are able to control the release of a hydroglycolic
extract of the Calendula officinalis flower. The main goal of
the present work was to carry out the preclinical evaluation of
SGC alone or loaded with the C. officinalis extract (SGC-E) in
a lagomorph model of full-thickness skin wound. A total of 39
rabbits were distributed in three groups, of 13 animals each.
The first group was used to compare wound healing by secondary
intention (control) with wound healing observed when
wounds were grafted with SGC alone. Comparison of control
wounds with wounds grafted with SGC-E was performed in
the second group, and comparison of wounds grafted with
SGC with wounds grafted with SGC-E was performed in the
third group. Clinical follow-ups were carried in all animals
after surgery, and histological and histomorphometric analyses were performed on tissues taken from the healed area
and healthy surrounding tissue. Histological and
histomorphometric results indicate that grafting of SGC alone
favors wound healing and brings a better clinical outcome
than grafting SGC-E. In vitro collagenase digestion data suggested
that the association of the C. officinalis extract to SGC
increased the SGC-E cross-linking, making it difficult to degrade
and affecting its biocompatibility.
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Atribución-NoComercial-CompartirIgual 2.5 Colombia