Aislamiento de hesperidina a partir de extractos etanólicos obtenidos de cáscara de mandarina y evaluación de su actividad antioxidante

Abstract

Colombia is a country characterized in the production of citrus fruits; however, this generates increased consumption that leads to the problem of disposal of solid organic waste. A scientific level has gained momentum in exploiting these wastes in order to recover substances of pharmaceutical, cosmetic and food interest among which include flavonoids. Hesperidin is a flavonoid found in the skin of citrus fruits and is known for having a wide range of pharmacological properties as an antioxidant, anti-inflammatory, anti-allergic and anti-carcinogenic. The main objective of this work was to isolate hesperidin from tangerine peel residues using the ultrasound as a technique for obtaining the ethanolic extracts, achieving an average yield of 7 % for the five samples made. The polyphenols content was quantified (18,74 to 24,66 mg AG/ g extract) and flavonoids (0,41 to 0,50 mg quercetin/ g extract) present in the extracts, also its antioxidant activity was evaluated by the radical cation (ABTS+.) method, obtaining values of 112,7 ± 3,1 to 174,2 ± 4,7 mmol Trolox/ kg sample. Hesperidin isolated did not show inhibition on the radical cation ABTS. Different spectrophotometric techniques, HPLC-DAD-UV / Vis, IR, NMR, and diffraction X-ray, using a standard reference, confirmed its structure.